湛水処理によるブドウの根のパーオキシダーゼ活性について   :   Effects of Flooding on the Peroxidase Activity in the Grape Roots 

作成者 西元, 直行, 大野, 正夫
作成者 (ヨミ) ニシモト, ナオユキ
作成者の別表記 NISHIMOTO, Naoyuki, OHNO, Masao
日本十進分類法 (NDC) 620
内容 ブドウ・デラウエアを供試し, 湛水処理を行ない, 根のパーオキシダーゼ活性の量的質的変化を観察した.(1)細根のパーオキシダーゼ活性は湛水処理の3日後と55日後に急激な増加を示し, 無処理区のものより高い活性量であった.側根では無処理区のものより低い活性量であった.(2)細根のタンパク質含量は, 処理の前半期において, 処理区の方が無処理区のものより少なくなった.しかし, 側根では処理の後半期において, 処理区の方が無処理区のものより多くなった.(3)比活性でみると, 細根において, 処理3日後と55日後に比活性が大きく増加した.側根の比活性は処理区の根が無処理区のものより低い値を示したが大差はなかった.(4)電気泳動のチモグラムパターンによると, 細根・側根ともに3〜4個のアイソザイムの存在を認め, 細根は処理により, 無処理区の根には現われないアイソザイムの存在が認められた.側根のパーオキシダーゼチモグラムパターンは無処理区との間に差は認められなかった.
Effects of Flooding on the Peroxidase Activity in the Grape Roots. N. NISHIMOTO and M. OHNO. Faculty of Horticulture, Chiba University, Matsudo, Japan. Tech. Bull. Fac. Hort. Chiba Univ., No.22: 1-6 1974. The flooding experiments were carried out from Sept. 1 using Delaware grapes grown in the 30cm pots. During the study, peroxidase activity and its isozyme in the roots were assayed. Peroxidase activity in flooded plant rootlets was increased significantly on both third and 55th days after flooding compared with that from unflooded plants, and the activity in the lateral roots from flooded plants was lower than that from unflooded ones except it was slightly higher on the third day. Protein content in flooded rootlets was lower in 19 days from the start of flooding than that from unflooded ones. The content in the lateral roots of flooded plants was much higher than that of unflooded lateral roots during the period from 19 to 29 days after flooding. Specific activity of peroxidase in rootlets was greatly affected by flooding, that is, specific activity was increased on both third and 55th days, however, the activity in lateral roots was unchanged. In disc electrophoresis, three or four isoperoxidases were found in both rootlets and lateral roots of grape plants. Apparent change in the pattern of zymogram of flooded rootlets was observed as compared with that of unflooded roots.

公開者 千葉大学園芸学部
コンテンツの種類 紀要論文 Departmental Bulletin Paper
DCMI資源タイプ text
ファイル形式 application/pdf
ISSN 0069-3227
NCID AN00142658
掲載誌情報 千葉大学園芸学部学術報告 Vol.22 page.1-6 (19750315)
情報源 The technical bulletin of Faculty of Horticulture, Chiba University
言語 日本語
著者版フラグ publisher

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