日本梨果実のポリフェノールオキシダーゼについて   :   Studies on polyphenol oxidase of Japanse pear fruits. 

作成者 小倉, 長雄, 鎌田, 庸宏, 佐藤, 隆英, 中川, 弘毅
作成者 (ヨミ) カマタ, ヤスヒロ, サトウ, タカヒデ
作成者の別表記 OGURA, Nagao, KAMATA, Yasuhiro, SATO, Takahide, NAKAGAWA, Hiroki
日本十進分類法 (NDC) 620
内容 1.9品種の日本梨果実のPP含量とPPO活性および破砕時の褐変度を検討した結果, 褐変の強さとPP含量・PPO活性量との間には直接的な関係は認められなかった.2.9品種のPPOアイソエンザイムをディスク電気泳動後の活性染色でみた所, 雲井で3種, 二十世紀で9種のバンドが観察され, 品種によりザイモグラムパターンが異なった.3.雲井を22℃に貯蔵し, 貯蔵中のPP含量, PPO活性変動と果実の褐変度合を調べた. PP含量は35日の貯蔵期間中ほぼ一定であった. PPO活性は貯蔵直後に減少したが14日目には回復し, 以後再び低下した.コアブラウンは14日目に出現し, 褐変は次第に拡がった.4.雲井の粗酵素はカテコールを基質とした時, Km値は19.5mM, 最適pHは5.8であった.クロロゲン酸に対して最も強く作用した.基質を替えたザイモグラムパターンより, クレゾラーゼ活性をもつアイソエンザイムの存在がみられた.
1) Polyphenol (PP) content and polyphenol oxidase (PPO) activity in nine varieties of Japanese pear were studied to elucidate the mechanism of browning of pear by crushing. Neither PP content nor PPO activity in pears were not correlated with their of browning degree. 2) PPO isoenzymes in nine varieties of pear were separated by means of polyacrylamide-gel electrophoresis and detected by zymography with catechol as substrate. Thus 3 kinds of isoenzyme in Kumoi and 9 kinds of it in Chojuro were observed, and their zymographic patterns were different according to the varieties of pears. 3) Changes in PP content and PPO activity of Kumoi during storage at 22℃ were investigated. PP content was constant throughout the period of storage. Although PPO activity decreased during the early stage of sorage, the activity recovered after 14 days. Core brown appered after 14 days of storage and the degree and area of browning increased under prolonged storage. 4) Crude PPO preparation of Kumoi had optimum pH5.8 and Km value was 19.2mM for catechol. Kumoi PPO showed the highest activity for chlorogenic acid and its isoenzyme having Rm 0.92 was suggested to be able to oxidize monphenol.

公開者 千葉大学園芸学部
コンテンツの種類 紀要論文 Departmental Bulletin Paper
DCMI資源タイプ text
ファイル形式 application/pdf
ISSN 0069-3227
NCID AN00142658
掲載誌情報 千葉大学園芸学部学術報告 Vol.32 page.45-49 (19831225)
情報源 The technical bulletin of Faculty of Horticulture, Chiba University
言語 日本語
著者版フラグ publisher

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