ホウレンソウ液体培養細胞のパーオキシダーゼ活性に及ぼす高温処理の影響   :   Effect of Heat Treatment on Peroxidase Activity in Cell Suspension Cultures of Spinach 

作成者 小倉, 長雄, 小林, 恭一, 佐藤, 隆英, 中川, 弘毅
作成者 (ヨミ) コバヤシ, キョウイチ, サトウ, タカヒデ
作成者の別表記 OGURA, Nagao, KOBAYASHI, Kyoichi, SATO, Takahide, NAKAGAWA, Hiroki
日本十進分類法 (NDC) 620
内容 1.ホウレンソウ液体培養細胞中のPO活性は, 継代後12日頃より急激に活性を増大し, 静止期に入る16日頃に最大の活性を持つようになった.2.培養8日目の細胞を37℃, 40分加熱処理後, 25℃で培養を続けると, 一時的に急激なPO活性上昇が生じ, 24時間後に最大に達し, 以後徐々に活性を減じ, 無処理で培養を続けた細胞の示す活性とほぼ等しくなり, 以降は無処理細胞と同じ変動を示した.3.高温処理により起るPO活性上昇は, 継代8日目以降の範囲でみた場合, 初期に処理するほど大きく生じるが, 最高値は無処理細胞が静止期に示す値とほぼ同じであった.4.シクロヘキシイミド, 6-メチルプリンの添加は高温処理により生ずるPO活性上昇を抑制した.
1) Peroxidase activity in cultured cells of spinach increased after 12 days of cultivation., The significant increase in peroxidase activity in suspension cells began before the stationary phase and reached maximum after 16 days. 2) When 8 day-old suspension cells were treated with high temperature at 37℃ for 40 min, peroxidase activity in the cells increased rapidly and reached maximum after 24 hrs. Thereafter the activity decreased gradually and became to coincide with that of untreated suspension cells. 3) When suspension cells were treated with high temperature at 37℃ for 8th day or later, the earlier we treated, the more the increase of heat-induced peroxidase activity occurred. However, the maximum values of the activity were almost equal to that in stationary phase of untreated suspension cells. 4) Cycloheximide and 6-methly purine inhibited the rapid increase of peroxidase activity in heat-treated suspension cells.

公開者 千葉大学園芸学部
コンテンツの種類 紀要論文 Departmental Bulletin Paper
DCMI資源タイプ text
ファイル形式 application/pdf
ISSN 0069-3227
NCID AN00142658
掲載誌情報 千葉大学園芸学部学術報告 Vol.35 page.15-18 (19850330)
情報源 The technical bulletin of Faculty of Horticulture, Chiba University
言語 日本語
著者版フラグ publisher

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